|
KMID : 0811720170210010117
|
|
Korean Journal of Physiology & Pharmacology
2017 Volume.21 No. 1 p.117 ~ p.124
|
|
|
Inhibition of ERK1/2 by silymarin in mouse mesangial cells
|
|
Youn Cha-Kyung
Cho Sung-Il
Lee Min-Young
Jeon Young-Jin
Lee Seog-Ki
|
|
|
Abstract
|
|
|
|
The present study aimed to show that pro-inflammatory cytokines [tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-1β] synergistically induce the production of nitric oxide (NO) production in mouse mesangial cells, which play an important role in inflammatory glomerular injury. We also found that co-treatment with cytokines at low doses (TNF-α; 5 ng/ml, IFN-γ; 5 ng/ml, and IL-1β; 1.25 U/ml) synergistically induced NO production, whereas treatment with each cytokine alone did not increase NO production at doses up to 100 ng/ml or 50 U/ml. Silymarin, a polyphenolic flavonoid isolated from milk thistle (Silybum marianum), attenuates cytokine mixture (TNF-α, IFN-γ, and IL-1β)-induced NO production. Western blot and RT-PCR analyses showed that silymarin inhibits inducible nitric oxide synthase (iNOS) expression in a dose-dependent manner. Silymarin also inhibited extracellular signal-regulated protein kinase-1 and -2 (ERK1/2) phosphorylation. Collectively, we have demonstrated that silymarin inhibits NO production in mouse mesangial cells, and may act as a useful anti-inflammatory agent.
|
|
|
KEYWORD
|
|
|
Cytokine, ERK1/2, iNOS, Mesangial cells, Nitric oxide
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|
|